The film, based on the hugely popular series of vampire novels by Stephenie Meyer, features a forbidden love between vampire Edward Cullen and human teen Bella Swan.
The big opening helps put Summit Entertainment on the map. The production company has only been in business since April 2007 and will be reaping huge profits on Twilight, which cost only $37 million to make.
"Teen girls rule the earth," says Paul Dergarabedian, president of Media By Numbers.
"If you look back at the Hannah Montana movie, how well that did, and now this movie, the teen girl audience will never be ignored again or underestimated. It was always teen boys who were the coveted ones, but someone finally caught on to the idea that girls love movies, too, and if you create something that they're into, that they're passionate about, they will come out in big numbers and drive the box office."
The rest of the Top Ten:
2. Quantum of Solace, $27.4 million
3. Bolt, $27 million
4. Madagascar 2: Escape 2 Africa, $16 million
5. Role Models. $7.2 million
6. Changeling, $2.6 million
7. High School Musical 3: Senior Year, $2 million
8. Zack and Miri Make a Porno, $1.7 million
9. The Boy in the Striped Pajamas, $1.67 million
10. The Secret Life of Bees, $1.28 million
Robert Pattinson and Kristen Stewart: The SheKnows Interview
The Twilight all-female creative team talks
Meet the Cullens!
Take the SheKnows Twilight trivia test
The Twilight soundtrack rocks
The SheKnows interview with Twilight author Stephenie Meyer
Do you have Twilight fever?
Enter to win a Twilight prize pack
Get the Twilight widget!
Chat with other Twilight fans on our Message Boards
And you'll see personalized content just for you whenever you click the My Feed .
SheKnows is making some changes!